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e-gel invitrogen manual

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e-gel invitrogen manualE-Gel agarose gels are ideal for analyzing PCR products, restriction digests, plasmid preparations, or for DNA fragment library analysis. E-Gel agarose gels are also suitable for a quick check of RNA samples. This superior sensitivity allows you to use lower amounts of sample, saving time and money. Each E-Gel agarose gel contains agarose, electrodes, SYBR Gold DNA stain, and a bufferless ion exchange system packaged inside a dry disposable cassette. E-Gel technology does not required any gel or buffer preparation or gel straining steps. Just load your samples and run. Live monitoring of DNA migration The E-Gel EX agarose gels are pre-stained with SYBR Gold II DNA gel stain with an excitation wavelength in the blue-light spectrum. When used on the E-Gel Power Snap Electrophoresis Device with integrated blue-light trans-illuminator, safe real-time monitoring of DNA or RNA migration is enabled without risk to eyes or possible damage of the sample, unlike UV illumination. Quick check of RNA samples Besides DNA analysis, E-Gel EX gels can also be used for fast analysis of RNA samples to check their integrity before proceeding with downstream applications. Easy access to the gel E-Gel cassettes are designed for convenient opening with a Gel Knife (Cat. No. EI9010), for easy excision of specific bands or transfer of the gel to a membrane for southern blot analysis. Nucleic acid markers For optimal DNA analysis on E-Gel agarose gels, we recommend specially formulated E-Gel 1 Kb Plus Express DNA Ladder (Cat. No. 10488091) or E-Gel 50 bp DNA Ladder (Cat. No. 10488099) or E-Gel Ultra Low DNA Ladder (Cat. No. 10488096). Our high-quality ready-to-use ladders are formulated using individual chromatography-purified DNA fragments and are suitable for storage at room temperature. The system consists of an electrophoresis device and a camera for fast and convenient E-Gel agarose gel separation and analysis.http://www.chriton.com/upimages/userfiles/cpim-exam-content-manual-free-download.xml

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The 11-well E-Gel EX agarose gels are available in 1, 2, and 4 gel percentages. E-Gel agarose gel selection guide Not for use in diagnostic procedures. With dry precast E-Gel agarose gel technology, you can run DNA samples in as little as 10 minutes and observe sample separation in real time. It is the only benchtop system that seamlessly integrates DNA sample separation and gel imaging into one workflow. Features of the E-Gel Power Snap Electrophoresis system include: Streamline electrophoresis and image capture with an all-in-one system Separate DNA in as little as 10 minutes with dry precast E-Gel agarose gels View and capture DNA samples in real time Designed for efficient and convenient benchtop use Small, fast, and powerful The all-in-one E-Gel Power Snap Electrophoresis System provides everything you need for DNA gel electrophoresis and gel imaging in a small lightweight benchtop system. The electrophoresis device includes a power supply, blue-light transilluminator, and amber filter. This enables gel separation and real-time sample tracking on E-Gel agarose gels pre-stained with SYBR Safe or SYBR Gold II DNA stains. The electrophoresis device arrives with pre-programmed protocols for each type of available E-Gel agarose gel. Instant image capture Capture high-resolution E-Gel agarose gel images on the go with the included easy-to-use E Gel Power Snap Camera. The camera is directly docked to the electrophoresis device, requiring no external power supply or connection to a desktop computer. This enables the freedom to capture images on the spot without leaving the bench. Made for convenience The E-Gel Power Snap Camera touch screen and onboard software provide an intuitive interface for quick gel image capture and editing. Store images using the Power Snap internal memory storage through months of camera use or easily transfer the images to an external USB memory device.http://dissanna.com/temp/fckeditor/cpim-exam-content-manual-2013.xml Unsurpassed convenience of dry precast E-Gel agarose gels Run DNA samples in as little as 10 minutes with dry, precast, pre-stained E-Gel agarose gels. The E-Gel dry pre-cast agarose gel technology provides speed and convenience by eliminating any need for gel preparation or staining. E-Gel agarose gels are available in a variety of gel percentages and with a choice of two DNA stains. The ethidium bromide-stained gels are not suitable for imaging with the E-Gel Power Snap Camera. Not for use in diagnostic procedures. A limited liability company corporated in England. Premixed buffer stocks, e gel manual invitrogen minicolumns to isolate plasmid DNA, and even agar plates prepared using premeasured capsules have all allowed Invitrogen iBlot Gel Transfer Device IB Description The iBlot Gel Transfer Device is a dry transfer device that performs western blotting transfer efficiently and reliably, within seven minutes, and without the need.Free shipping for many products. Specially designed for running E-Gel 48, E-Gel 96, E-PAGE and E-PAGE gels Manufacturer: EBM Catalog No. EBM View and Download Invitrogen A quick reference online. Mini Gel Tank. A laboratory equipment pdf manual download. Page QUICK REFERENCE Mini Gel Tank Cat. A Publication Part No. Instructions for using the Mini Gel Tank to perform electrophoresis are described below. The Thermo Invitrogen E-Gel Power Snap Electrophoresis System offers speedy electrophoresis in as little as minutes, that you can watch in real time. This benchtop electrophoresis system is ideal for DNA sample loading, gel imaging, and lets you simplify your work flow. Technology provides speed and convenience by eliminating any need for The Invitrogen E-gel Agarose Electophoresis System makes traditional agarose gels almost a thing of the past by using pre-cast gels. No more preparation of buffer, weighing out and melting agarose, dealing with toxic ethidium bromide, pouring the gel and waiting for it to set, etc.http://www.raumboerse-luzern.ch/mieten/bose-stereo-system-manual E-Gel precast gels are self-contained and ready for use, with the agarose and the DNA stain packaged in a disposable cassette. There are no gels to pour, buffers to make, staining or destaining steps to perform, or gel boxes to. For more information visit The used Invitrogen E-gel Safe Imager Real-time Transilluminator was tested in our laboratory and found to be in good state. Invitrogen E Gel Power Snap Camera Features. Invitrogen E gel iBASE Power System for sale online. Invitrogen Mother E Base Device. INVITROGEN A25977 QUICK REFERENCE Pdf Download. DNA separation E Power Snap. Thermo Invitrogen E Gel Power Snap Electrophoresis. Invitrogen E Gel PowerBase Version 4. Agarose Electrophoresis Gels Fisher Scientific. E Power Snap Elektrophoresesystem. Imager Life Technologies E PowerPac Basic Power Supply Instruction Manual. 1252 Used Invitrogen E gel Safe Imager Real time. Invitrogen iBlot 2 Gel Transfer Device IB21001 ANDbio. E Gel Electrophoresis System Thermo Fisher Scientific. Manual Invitrogen E gel Safe Imager Real time. View the product page for possible alternatives. E-Gels are available in two pack sizes-a Starter Pak, and 18-gel multi-paks.Not for use in diagnostic procedures. All Rights Reserved. All usage must comply with product instructions. Store at room temperature. Promotion code must be presented at the time of order. Discount applies to the list price in effect at the time the order is received. Promotion cannot be combined with other discounts or promotions. Other restrictions may apply. Your commerce experience may be limited. Please update your browser to Internet Explorer 11 or above. When you select your country, you agree that we can place these functional cookies on your device. After that, you will need to contact Customer Service to unlock your account. Please try again or contact Customer Service. Please request another reset link. Please try again or contact Customer Service.http://kampongtourist.com/images/canon-pixma-ip400-manual.pdf A verified email address is required to access the full functionality of your Promega.com account. Please try again or contact Customer Service. Please try again or contact Customer Service. Please try again or contact Customer Service. Please check your network settings and try again. Please try again or contact Customer Service. All five BenchTop DNA Markers are compatible with both systems, offering researchers flexibility and convenience in the use of these molecular weight markers. Prior to the discovery that DNA fragments could be separated by size via gel electrophoresis, scientists used methods like sucrose density gradients to determine the fragment lengths of DNA in their sample. In 1968, Takahashi et al.Commercially available DNA ladders, or markers, aided scientists in accurately sizing their DNA fragments. These markers were frozen fragments of DNA, and for use on agarose gels, required the addition of a loading dye prior to loading into the gel. DNA markers also have evolved, with options for a variety of fragment sizes, premixed loading dye, and stability at room temperature. The ladder was run in duplicate and visualized with ethidium bromide. All known molecular weight bands can be observed on both gel types.All known molecular weight bands can be observed on both gel types.The direct-loading capability, coupled with room temperature stability and precast gel compatibility make the BenchTop DNA Markers a convenient and reliable means to accurately determine the size of DNA fragments on an agarose gel. Updated 2011. Accessed Month Day, Year. E-Gel Ultra Low Range DNA Ladder consists of nine individual chromatography-purified DNA fragments with a reference band at 100 bp for easy orientation. E-Gel Ultra Low Range DNA Ladder is specifically formulated for optimal performance on pre-cast E-Gel agarose gels. Highlights of E-Gel Ultra Low Range DNA Ladder: Performance—optimized for use on 4 E-Gel agarose gels Sharp, clear bands—chromatography-purified fragments for consistent and reliable results, stable at room temperature up to six months Ready to use—premixed with loading buffer Convenient—provided with 1X E-Gel Sample Loading Buffer Precise—an exact amount of DNA in each band Not for use in diagnostic procedures. All Rights Reserved. This includes personalizing content and advertising. To learn more and manage cookies, please refer to our Cookie Statement. For maximum convenience and value, columns and buffers are also available separately. Learn more and request a sample. Supporting COVID-19 Research Our new RUO kit, the SARS-CoV-2 Rapid Colorimetric LAMP Assay Kit, enables simple, visual detection of isothermal amplification of SARS-CoV-2 nucleic acid. The DNA Ladder consists of proprietary plasmids, which are digested to completion with appropriate restriction enzymes to yield 11 bands suitable for use as molecular weight standards for fast electrophoresis systems as well as standard electrophoresis. The digested DNA includes fragments ranging from 50 base pairs to 10 kilobases. The 1 kb fragment has increased intensity to serve as reference band.The best separation occurs on a 1.2 agarose gel. Below 1, the 50 bp fragment may not separate from the 150 bp fragment. The Fast DNA Ladder is not intended for precise quantification of DNA mass but can be used for approximating the mass of DNA in comparably intense samples of similar size.What percentage of agarose gel should I use. Why is my DNA Ladder floating out the wells and Why are there no bands visible in my DNA ladder gel lane? Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.Fast DNA Ladder This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals. However, this research should always be done in safe and ethical manner. The purchase of this product conveys to the user the non-transferable right to use the purchased amount of product for Research Use Only. Commercial use of this product may require a license from New England Biolabs.Please sign back in to continue your session. Adding products to your cart without being signed in will result in a loss of your cart when you do sign in or leave the site. The procedure can be fully automated on the QIAcube Connect. For optimal results it is recommended to use this product together with QIAvac 24 Plus. This product is not intended for the diagnosis, prevention, or treatment of a disease. Samples were analyzed on a 3.5 high-resolution agarose gel in TAE buffer. M: pTZ- Hin fI markers. DNA fragments smaller than 70 bp or larger than 10 kb should be extracted with the QIAEX II Gel Extraction System. Silica-membrane technology eliminates the problems and inconvenience associated with loose resins and slurries. Specialized binding buffers are optimized for specific applications and promote selective adsorption of DNA molecules within particular size ranges. Gel loading dye To enable faster and more convenient sample processing and analysis, gel loading dye is provided.Nucleic acids adsorb to the silica membrane in the high-salt conditions provided by the buffer. Impurities are washed away and pure DNA is eluted with a small volume of low-salt buffer provided or water, ready to use in all subsequent applications. Handling QIAquick spin columns are designed to provide two convenient handling options. The spin columns fit into a conventional table-top microcentrifuge or onto any vacuum manifold with luer connectors, such as the QIAvac 24 Plus with QIAvac Luer Adapters. Note: Remove as much of the supernatant as you can without disturbing the cell pellet. Chloroform is a carcinogen. Wear gloves, goggles and lab coat, and keep tubes capped tightly. To be safe, work in the hood if possible. Again, mix well by inverting the tube. Keep the tube at -20 degree for at least 30 min (the longer the better) and then spin it down (see Steps 15-16). You should see DNA pellet. It looks transparency when it is wet and turns to white when it becomes dry. Carefully discard the supernatant and air-dry the DNA pellet (tilt the tube a little bit on paper towel).Note: Large amounts of RNA will be present in the DNA sample. So, for subsequent reactions, for example, to digest plasmid DNA, add 1-5 ?l (1 mg ml -1 ) RNAase to the digestion solution to completely remove RNA. Or, add RNAase directly to lysis buffer with a final concentration of 1 mg ml -1. Note: we expect to see bands with smear patterns from high to low MW range, although most of DNA fragments are accumulated at high MW on the gel. So, if you see most of DNA fragments are small, very likely your DNA got degraded. Does the temperature of ethanol matter? 4. Why resuspend extracted DNA in TE buffer and not in water. What is EDTA? 5. What chemical should be used to get rid of the RNA contaminant in the above DNA preparation. How does it act?It might answer your queries It worked for me. Thanks to Fanglian He.Thank you in advance for the respond and providing the protocol.Proteinase K has to be added freshly. Thus, lysis buffer containing proteinase K should not be used next day. Otherwise, it can be kept at room temperature at least 6 months. (ii) Will it be fine if I use MH broth instead of LB broth. I never tried MH broth. But, I think it should be fine if your bugs grow happily in the media. (iii) How much TE buffer do you add for step 18? 50 microliter. (iv) If I were to determine the concentration of DNA eventually using spectrophotometry, how can I get the dilution factor for the amount of TE buffer added in (iii). Well, depending on how you would do the dilution, I guess you have to do your own math to get the answer. --Fanglian How long do I need for the incubation?Because i want to remove the RNA. Sorry i can understand the explaination in the note on step no8Good luck, Fanglian Thus, Bio-protocol does not publish this type of protocols as a separate publication. You could either find the instructions on the company websites or contact them directly. Good luck! FanglianDoes this protocol yield high molecular weight DNA. I am interested in extracting like 80kb and above. Also, after the lysis step, is the lysate not too thick (mucous) to handle and even seperate the proteins out after phenol-chloroform? And, the lysate was not too thick to handle in my hands. Good luck! FanglianChikara Kaito at the University of Tokyo for help with your question. The method is written on page 589-590. Here, I want to thank Prof. Chikara Kaito again for his prompt response and helpful information. Good luck, --FanglianKeep the tube at -20 degree for at least 30 min (the longer the better) and then spin it down. You should see DNA pellet (it looks transparency when it is wet, and turns to white when it becomes dry). --FanglianRegarding your 2nd question, unfortunately, I did not record the time when I ran my genomic DNA from E coli. But, my impression was that it should not take too long. In case your sample was too condensed, you might run your samples with 1 or 2 more dilutions. --FanglianPlease help to mePlease use Bio-protocol RaP service ( ) to request such a protocol. Thanks, FanglianBut, I found a good example from this link, (Figure 2 is copied below) Clearly the process can work without but do you know how beneficial it is to use? Thanks!Any references? Thanks, Fanglian From the original paper about CTAB method by Kate Wilson ( ), it seems that CTAB can help to remove polysaccharide contamination in DNA prep.Thus, glucose may not have to be included in the resuspension buffer. I never tried the one without glucose, though. So, please share your experience with us if you try it without glucose. Good luck, FanglianIn reference point of view.In this protocol, TE is added to resuspend the DNA pellet after ethanol precipitation. Hope this helps. Good luck, Fanglian Will the protease or SDS denature or degrade my RNAse.Should all of the solutions were scaled increased. Thanks in advance! Should all of the solutions were scaled increased. Thanks in advance! Should all of the solutions were scaled increased. Thanks in advance! Should all of the solutions were scaled increased. Thanks in advance! Should all of the solutions were scaled increased. Thanks in advance! Small confusion explain me. Thanks in advance Suresh G Hope your question is clarified. Kind Regards Ken While I never tried it, theoretically I think it should work. If you try it with a high-copy plasmid, you may be able to distinguish plasmid DNA from genomic DNA on a DNA gel if it works. I am just student who has studied this part and this experiment i did was my first experiment.Hope you would find them helpful. Good luck, Fanglian There seems to be a lot of carbohydrate in the prep. thanksBut, I never tried to remove the carbohydrate contamination from my genomic DNA prep. I think you may have to use some commercial kits to get more pure genomic DNA. Good luck, Fanglian For some related references, you may find it at --Fanglian Please see below for some recommended references related to above suggestions: 1. Maniatis T., E.F. Fritsch, and J. Sambrook (1982) Molecular Cloning A Laboratory Manual, Cold Spring Harbor Laboratory, Cold Springs Harbor, NY. 2. --Fanglian For genomic DNA, most of them are very big and do not migrate far from well. And, since they often consist of DNA fragments with different sizes, you can bands with the big range of sizes (bands are smear if many got degraded). For plasmid DNA, when they are uncut, they often appear more than one band on the gel (due to different topology). To confirm the plasmid DNA of your interest, you can cut the DNA with known restriction enzymes before run the gel. For RNA, I do not have much experience on running specific RNA samples on agarose gels (may need denaturing agarose gel). But, for RNA contamination found in your DNA prep (without RNase treatment), RNA bands (often very bright) migrate toward the bottom of the gel. Hope this could be helpful. If you do see it, you can keep the tube at -20 degree overnight, which will increase the yield. Another way to increase the yield is that you could use more cell culture (e.g. 5 ml). Good luck! But, RNase is used to digest RNA contamination in genomic DNA prep. But, RNase is used to digest RNA contamination in genomic DNA prep. I did not use it in my experiments. But, it might help to increase the DNA yield by using sodium acetate. EDTA is used to to inhibit DNases. Ethanol is used to precipitate DNA. So, if you see most of DNA fragments are small, very likely your DNA got degraded. Two possible reasons that may cause DNA degradation in this protocol: make sure do not vigorously vortex and pipet of DNA solutions at step6 or subsequent steps. It may have some DNases contamination in your sample. DNases released from the lysed cells are supposed to be removed by phenol and chloroform extraction (involves protein denaturation too). You can try to check your DNA on the gel before you treat it with RNase. Hope I answered your questions. Another option is to try some commercial kits, like QIAamp DNA Mini Kit. They should be good, but expensive. (I personally nerve tried them though) By using our website, you are agreeing to allow the storage of cookies on your computer. Sorted nuclei were sheared by Covaris E220 and used for ChIP for overnight. Immunoprecipitates were washed and subjected to elution and reverse crosslinking. After ligation, DNA was eluted and amplified by PCR for 14-16 cycles using the PfuUltra II Hotstart PCR Master Mix. DNA sized between 250 and 600bp was selected by gel electrophoresis and extraction using E-Gel EX Agarose Gels (Invitrogen) and MinElute Gel Extraction kits (Qiagen). Libraries were analyzed using the Qubit and Agilent Bioanalyzer, pooled at a final concentration of 12pM and sequenced on a HiSeq2500 or NextSeq500 system. Duplicates and low quality reads were removed by Picard ( ) Reads were processed using samtools (Li et al., 2009) Peaks were called by MACS2 (Zhang et al., 2008), and low peaks (4 Reads were assigned to peaks, normalized and quantified using featureCounts and EdgeR. Low signal peaks (log2 CPM For ChIP-seq track visualization, reads were processed to the BigWig file format using bedtools (Quinlan and Hall, 2010) and bedGraphToBigWig (Kent et al., 2010). Processed Bigwig files were viewed in WashU Epigenome Browser (Zhou and Wang, 2012). ChIP-seq peak heatmaps and distribution plots were generated with the normalized BigWig files within 10kb genomic regions surrounding peaks by using deepTools2 (Ramirez et al., 2016). Overlaps between H3K27ac and H3K4me1 were determined using bedtools. Peaks were assigned to genomic annotations and genes with HOMER (Heinz et al., 2010), and GO pathway analysis was performed by DAVID Bioinformatics Resources 6.8. Motif enrichment analysis was conducted using FIMO from the MEME package (Grant et al., 2011) with the JASPAR database (Mathelier et al., 2014) genome build: mm10 processed data files format and content: bigwig. Voce deve ativar o Javascript no seu navegador para utilizar as funcionalidades deste site. Simply connect the AC adaptor provided and plug into an electrical outlet. View program selection and running time on the easy-to-read LCD display. Preset programs are available for various gel types or you can manually set your own run times. Unlike UV, blue light causes minimal damage to DNA, offering improved safety for the user and enhanced cloning efficiency of any DNA retrieved from the gels. Not for use in diagnostic procedures. The systemis designed to be used without liquid buffer.IMORTANT! Ethidium bromide, EtBr, is very toxic, strongly mutagenic and a possiblecarcinogen. EtBr goes straight through latex gloves, so never use these whenworking with EtBr. Vinyl gives some protection, but not enough. Please always usenitrile gloves to give maximum protection.Always wear a lab coat when working with EtBr. Change the lab coat if you get EtBron it and put the dirty one in the laundry-bin.Remember that the whole area, hood, bench, sink and camera are EtBrcontaminated.Make sure you follow all the instructions given when handling this chemical. G7008-02, Invitrogen ) containing EtBr are used.2. All handling of gel s should be done in a fume hood (except when taking thephotograph).3. Load each gel within 30 minutes of removing gel from the package and run within15 minutes of loading. The gel must not dry out before use.4. Remove gel from the package and remove red plastic comb from the gel. LM 025 Page 2: DEPT OF EVOLUTIONARY BIOLOGY, UPPSA Thank you, for helping us keep this platform clean. The editors will have a look at it as soon as possible. You can disable the usage of cookies by changing the settings of your browser. By browsing our website without changing the browser settings you grant us permission to store that information on your device. This separation is achieved by the nuclear envelope, which controls the exchange of macromolecules through nuclear pores 2. During mitosis, however, the nuclear envelope in animal and plant cells disassembles, allowing cytoplasmic and nuclear components to intermix 3. When the nuclear envelope is reformed, cytoplasmic components are removed from the nucleus by receptor-mediated transport through nuclear pores 2. Here we show in HeLa cells that large cytoplasmic components are displaced before nuclear envelope assembly by the movement of chromosomes to a dense cluster. This clustering occurs when chromosomes approach the poles of anaphase spindles, and is mediated by a microtubule-independent mechanism that involves the surfactant-like protein Ki-67. Ki-67 forms repulsive molecular brushes during the early stages of mitosis 8, but during mitotic exit the brushes collapse and Ki-67 promotes chromosome clustering. We show that the exclusion of mature ribosomes from the nucleus after mitosis depends on Ki-67-regulated chromosome clustering. Thus, our study reveals that chromosome mechanics help to re-establish the compartmentalization of eukaryotic cells after open mitosis. We will provide the code upon request. Dashed lines indicate fractions loaded on the gel blotted in e.Single z -slice shown.Normalization to average value of metaphase time point.Imaging as in a. d, Quantification of H3(pS10) mean fluorescence intensity and chromatin area in spindle-less mitosis, as shown in ( c ), demonstrates that histone 3-serine 10 was efficiently dephosphorylated in flavopiridol-induced mitotic exit and chromosomes cluster to a degree comparable to that of normal late anaphase. Normalization to average value of prometaphase time point. Yellow line indicates convex hull around chromosomes, single z -slice shown. Time is relative to onset of clustering.Individual cell curves were aligned based on half-maximum value of convex hull area. Normalization to average of first 4 time points.Time relative to nuclear envelope breakdown (NEBD), single z -slice shown. Representative example of 14 cells shown.Chromosome arms extend out of the metaphase plate before flavopiridol addition, but densely cluster on the metaphase plate 8 min after flavopiridol addition.Values normalized to average of all frames before first drug treatment.White dashed lines indicate chromosomal areas, single z -slice shown.Single z-slice shown. Sample 3 was lysed after 48 h, whereas sample 2 was additionally FACS sorted for the 10 brightest cells which we estimated to represent the population of cells that suppress the Ki-67-knockout individualization failure phenotype.Single z -slices shown.Cells were treated with trichostatin A 2 h before imaging to rescue the Ki-67-knockout individualization failure phenotype in Ki-67-KO cells. Representative examples stained with SiR-Hoechst, single z -slices shown.Showing one example of two biological replicates.Quantification of chromosome convex hull area, normalized to pre-flavopiridol time points ( c ) and representative examples stained with SiR-Hoechst ( d ).Ki-67(RASA) was tagged by mCherry and eGFP on either protein end, respectively, and expressed in HeLa cells bearing the endogenous RASA mutation in all three copies of Ki-67.Single z -slice is shown. Values normalized to average of wild-type 5 min time point. Showing combined data of two independent biological replicates.Video 2 Mature ribosome exclusion during anaphase Confocal imaging of live HeLa cell expressing L10-EGFP (green) and H2B-mCherry (magenta) progressing through anaphase. Video 3 Chromosome motility during early mitosis and during mitotic exit in the clustered state. Live mitotic HeLa cell stably expressing H2B-mCherry (magenta) and CENP-A-EGFP (green) was imaged in the presence of nocodazole before (left panel) and after flavopiridol addition (right panel), time relative to flavopiridol addition.